Figure 3.

Temperature dependence of $T\Delta S_i^{\ast }$ and $\Delta H_i^{\ast }$ (same arbitrary units as in Fig. 2) for fusion of control vesicles (fusion induced by the 5% (w/w) PEG). $T\Delta S_i^{\ast }\left(=-a_{i}T-2b_i{T}^2-3c_i{T}^3\right)$; solid line) and $\Delta H_i^{\ast }\left(=y_{0,i}-b_i{T}^2-2c_i{T}^3\right)$; dotted line) for the formation of (A) I₁ state, (B) I₂ state, and (C) pore for control vesicles. Insets show $\Delta C{p}_i^{\ast }\left(=-2b_{i}T-6c_i{T}^2\right)$ versus temperature for each step. $\Delta C{p}_i^{\ast }$ does not depend on the unknown constant A, so $\Delta C{p}_i^{\ast }$ units are not arbitrary. Parameters for these expressions as well as their variances are given in Table S3.