Figure 1.
Kinetic analysis of neutrophil exocytosis. Neutrophils were isolated from bone marrow of wild-type and Rac2-/- mice. 1 × 106 cells were incubated with 5 μM CB for 5 min followed by 5 μM fMLF. At the indicated times samples were taken and cell-free supernatants were analyzed for MPO, an enzyme contained in primary granules. Exocytosis was measure as the % MPO release, calculated as a ratio of total cellular MPO. A. Shown is a representative result from a typical degranulation assays. B. Pre-incubation of BMN with 10 μM cycloheximide for 1 hr at 37°C prior to CB/fMLF stimulation does not affect degranulation (n = 3).