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Microscopic analysis of granules and F-actin. Cytospins of BMNs freshly prepared from wild-type and Rac2-/- mice showing F-actin structures stained with phalloidin (red) and primary granules stained with CD63 (green). Cortical actin staining is more intense in unstimulated Rac2-/- cells compared to wild-type cells (unstim). The F-actin ring structure is dispersed in both cells following 15 min of CB/fMLF stimulation (stim). 60x/1.4 numerical aperture objective; bar = 5 μm.
