Fig. 7.

CRA-induced mucus secretion and expression of proinflammatory molecules found in inflammatory zone 1 (FIZZ1) and intelectin-1 are reduced by inhibition of PI3K p110δ activity. Lung sections from control and CRA-exposed mice treated with IC87114 or vehicle alone were evaluated for mucus secretion based on periodic acid-Schiff's (PAS) staining as well as expression of FIZZ1 and intelectin-1 by immunohistology. Representative microscopic images (left, magnification ×200) and combined quantitative data (right) of 3–6 airways from control group (n = 5) and 8–13 airways from CRA-exposed groups (n = 6–7) are shown. Data represent means ± SE. *P < 0.02 for PAS-positive staining and expression of FIZZ1 and intelectin-1 for control vs. CRA-exposed vehicle-treated mice; **P < 0.05 for PAS-positive staining and intelectin-1 expression and <0.01 for FIZZ1 expression comparing CRA-exposed IC87114-treated mice with vehicle-treated group.