Fig. 9.
Denopamine transactivates β2-ARs to promote lung fluid clearance. A: T1 cells were pretreated with 50 nM of ICI 118,551, a highly selective β2-AR antagonist, and with subsequent application of denopamine to the saline bath after a control recording period. Arrows indicate closed state, and downward deflections from the closed state depict entry of Na ions. #Break in continuous cell-attached patch-clamp recording. B: results for 4 independent observations shown on dot-plot graphs with y-axis = ENaC activity (Po) in T1 cells pretreated with 50 nM ICI 118,551 and then stimulated with 20 μM denopamine. C: lung fluid clearance in mice receiving an IP injection of 20 μM denopamine (■) and pulmonary edema in 50 nM ICI 118,551 and 20 μM denopamine (▴)-treated animals; asterisk denotes statistical significance (P < 0.05).