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Effective stiffness of BLMVEC glycocalyx measured by RICM utilizing borosilicate glass probes (diameter ≈ 17.3 μm) after hyaluronidase (HAse), heparinase III (HSIIIase), and pronase treatments. HAse-treated BLMVEC glycocalyx (n = 12) and pronase-treated BLMVEC monolayers (n = 8) showed significant changes in effective stiffness (P < 0.05) compared with the controls (n = 17); HSIIIase-treated BLMVEC glycocalyx (n = 7) was not statistically different from controls.
