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Effective stiffness of BLMVEC glycocalyx measured by RICM utilizing borosilicate glass probes (diameter ≈ 17.3 μm) after actin disruption with 100 nM cytochalasin D treatment (30 min). Cytochalasin D treatment of confluent monolayers resulted in increased stiffness. The confocal images (bottom) show the staining of the actin cytoskeleton for control and 100 nM cytochalasin D-treated monolayers.
