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Effective stiffness of BLMVEC glycocalyx measured by RICM utilizing borosilicate glass probes (diameter ≈ 17.3 μm) in presence of 0.1%, 1.0%, and 4.0% BSA in the medium. Effective glycocalyx stiffness was highest with 1.0% BSA (P < 0.05) when compared with 0.1% and 4.0% BSA samples. The representative RICM images (bottom) show that the glycocalyx thickness increases with the addition of BSA.
