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. 2011 Dec 7;122(Pt 7):349–359. doi: 10.1042/CS20110378

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) C_t values for the housekeeping gene β-actin reveal identical amplification efficiency for both BeWo and JEG-3 cells. (B) Melting curve analysis for β-actin in both cell lines, demonstrates a single melting peak at identical temperature. (C) Q-PCR demonstrates that DEPTOR is significantly expressed in BeWo cells when compared with JEG-3; **P<0.01.