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. 2011 Dec 7;122(Pt 7):349–359. doi: 10.1042/CS20110378

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Densitometric analysis of DEPTOR revealed a notable up-regulation at the protein level in BeWo cells when compared with JEG-3, mirroring the changes seen at gene level using Q-PCR. (B and C) Immunofluorescent analysis of DEPTOR in BeWo and JEG-3 cells respectively revealed cytoplasmic localization in both cell lines. Circles in (B) and (C) indicate the same amount of cells used for densitometric analysis as in (A). Images were analysed using Image J 1.34s image-analysis software (**P<0.001). Results are presented as means±S.E.M. (D and E) Negative controls of (B and C) where the primary antibody has been omitted, demonstrating staining specificity.