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. 2011 Dec 7;122(Pt 7):349–359. doi: 10.1042/CS20110378

Table 2. Primers used for Q-PCR studies.

Gene Expected size (bp) Primer sequence
DEPTOR 202 Forward, 5′-CACCATGTGTGTGATGAGCA-3′
Reverse, 5′-TGAAGGTGCGCTCATACTTG-3′
Rictor 117 Forward, 5′-GGAAGCCTGTTGATGGTGAT-3′
Reverse, 5′-GGCAGCCTGTTTTATGGTGT-3′
Raptor 170 Forward, 5′-ACTGATGGAGTCCGAAATGC-3′
Reverse, 5′-TCATCCGATCCTTCATCCTC-3′
mTOR 135 Forward, 5′-TGCCAACTATCTTCGGAACC-3′
Reverse, 5′-GCTCGCTTCACCTCAAATTC-3′
β-Actin 216 Forward, 5′-AAGAGAGGCATCCTCACCCT-3′
Reverse, 5′-TACATGGCTGGGGTGTTGAA-3′