Figure 2.

Knockdown of Akt2 decreased IKKε level in breast cancer cells. HCC1937 (A) or MCF-10CA1a (B) cells were transfected with indicated siRNAs, treated with or without 1 nM TNF, and Western blot analyses were carried out with the indicated antibodies, as described in the Materials and Methods. The results are representative of 2 individual experiments.