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Overexpression of Akt2 increased IKKε level. (A) MDA-MB-231 cells stably transfected with pBabe or pBabe-Akt2 were treated with or without 1 nM TNF, and Western blot analyses were carried out with the indicated antibodies. (B) Densitometric quantification of IKKε level from 3 independent experiments normalized with actin. The data represent the fold change in IKKε level compared with untreated vector-transfected cells.
