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. 2011 Nov;2(11):1044–1050. doi: 10.1177/1947601912444604

Figure 5.

Figure 5.

Akt2 induced IKKε by activating NF-κB. (A) MDA-MB-231 cells transfected with Akt2 or control nontargeting siRNA were transfected with 3X-κB construct and Renilla luciferase and treated with or without 1 nM TNF, as described in the Materials and Methods. Luciferase activity was determined, and the relative luciferase units were normalized and represented as fold change. *P < 0.05. The results are representative of 3 independent experiments. (B) The MDA-MB-231/pBabe and MDA-MB-231/Akt2 cells were transfected with 3X-κB construct and Renilla luciferase and treated with or without 1 nM TNF, as indicated in the Materials and Methods. The results are representative of 2 independent experiments. (C) The MDA-MB-231/pBabe and MDA-MB-231/Akt2 cells were transfected with p65/RelA siRNA or nontargeting control siRNA and treated with 1 nM of TNF for 24 hours and processed. Western blot analyses were carried out with the indicated antibodies. The results are representative of 2 independent experiments.