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. 2012 Jun 20;102(12):2808–2817. doi: 10.1016/j.bpj.2012.05.019

Figure 2.

Figure 2

(A) Comparison of the Δnw numbers for the DNA structure formations consisting of the same basepairs. The data for the double-stranded duplex obtained in i), 1 M NaCl, ii), 10 mM MgCl2, and iii), 1 mM [Co(NH3)6]Cl3 are also compared. For the experiments using MgCl2 and [Co(NH3)6]Cl3, the buffer solutions containing 25 mM HEPES (pH 7.0) and 0.1 mM Na2EDTA were used. (B) The Δnw numbers for the 11-mer duplexes having different basepairs in the middle and the 9-mer duplex without trinucleotide interactions in the middle of the 11-mer sequence. F and X indicate the tetrahydrofuran abasic site and Cnaph, respectively. (C) Two types of conformations of the tandem G/A mismatch formed in a DNA sequence 5′-pyrimidine-GA-purine-3′ (type I) and 5′-purine-GA-pyrimidine-3′ (type II). The Δnw numbers of the DNA duplexes forming the type I and type II conformations and those forming corresponding fully matched duplexes or the single G/A mismatch sites are compared.