Table 1.
DNA sequence | Stem length (bp) | Loop sequence | –ΔG° (kcal mol−1)∗ | –Δnw |
---|---|---|---|---|
1a | 5 | C6 | 1.72 | 56 ± 2 |
1b | 5 | C6 | 1.76 | 59 ± 3 |
2a | 6 | T6 | 1.87 | 73 ± 3 |
2b | 6 | T6 | 1.90 | 70 ± 3 |
3a | 5 | T6 | 2.48 | 68 ± 3 |
3b | 5 | T6 | 2.43 | 70 ± 3 |
4a | 5 | A6 | 1.63 | 50 ± 2 |
4b | 5 | A6 | 1.68 | 51 ± 2 |
5a | 5 | hEG† | 2.72 | 50 ± 4 |
6a | 5 | GA3 | 3.23 | 67 ± 4 |
7a | 6 | C6 | 1.28 | 58 ± 2 |
8a | 6 | A6 | 1.21 | 56 ± 2 |
9a | 8 | No loop | 5.26 | 53 ± 10 |
9b | 8 | No loop | 4.98 | 49 ± 13 |
10a | 8 | No loop | 12.3 | 102 ± 12 |
10b | 8 | No loop | 11.7 | 90 ± 6 |
ΔG° was calculated at 37°C and the error value for ΔG° are smaller than 6%.
hEG indicates the hexa(ethylene glycol) linker.