FIG. 3.

Insulin transcription increases in Akita testes and sperm. A: Differential expression of ins1 and ins2 by RT-PCR. Both transcripts are present in the pancreas, while only ins2 is present in the testes. B: qRT-PCR demonstrates expression of both insulin genes as decreases in the Akita pancreas and increases in Akita homozygous testes. C: mRNA for pdx-1, a transcription factor for both insulin genes, decreases expression in Akita pancreas and yet also increases in Akita homozygous testes, following the same trend as ins2 (9-week-old mice; n = 3). *P < 0.05, **P < 0.01. hom or homo, homozygous; het, heterozygous; WT, wild type. (A high-quality color representation of this figure is available in the online issue.)