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. 2012 Jun 15;61(7):1734–1741. doi: 10.2337/db11-1201

FIG. 3.

FIG. 3.

Knockdown of Kcnj15 increased insulin secretion in vitro. A: Agarose gel electrophoresis of the products and real-time PCR results generated by RT-PCR using primers specific for rat Kcnj15 and β-actin. INS1 cells were transfected with PBS, control siRNA, or Kcnj15 siRNA. B: Kcnj protein levels in cultured INS1 cell lysates with and without inactivation of Kcnj15 detected by Western blotting. INS1 cells were transfected with PBS, control siRNA, or Kcnj15 siRNA. C: The protein levels of insulin and actin in cultured INS1 cell lysates detected by Western blotting. D: Relative insulin secretion with and without inactivation of Kcnj15 in INS1 cells at 5 and 25 mmol/L glucose. Results are expressed as a percentage of the value secreted with 5 mmol/L (0.98 ± 0.04 mg/mg protein) as a control. Values are mean ± SEM (n = 5).