FIG. 5.

Interactions of Kcnj15 and CsR in INS1 cells. A: Coimmunoprecipitation of CsR with Kcnj15 or Kcnj11 from INS1 cell lysate. The INS1 cell membrane and cytosol fractions were processed for immunoblotting with antibodies against Kcnj15, Kcnj11, or CsR to detect the endogenous proteins. B: Cell lysates were used for immunoprecipitation with the anti-CsR antibody. The immunoprecipitated samples were processed for immunoblotting with antibodies against Kcnj15, Kcnj11, or CsR. C: Relative insulin secretion with and without inactivation of Kcnj15 and/or CsR in INS1 cells at 5 and 25 mmol/L glucose. Results are expressed as a percentage of the value secreted with 5 mmol/L as a control. Values are mean ± SEM (n = 5).