Table 4.
Median quantitative ratios for methylation of genes in NSCLC and adjacent non-malignant tissue
| Methylated gene | Median quantitative ratio for NSCLC (n=40) | Median quantitative ratio for adjacent non-malignant lung (n=40) | Median quantitative ratio for PBMCs from cancer-free individuals (n=40) | % Samples +ve for methylation in NSCLC | Cutoff value |
|---|---|---|---|---|---|
| 3-OST-2 | 14.19(0–975) | 0(0–4.1) | 0(0–0) | 28/40(70%) | 4.1 |
| RASSF1A | 0.69(0–883.10) | 0(0–4.013) | 0(0–0) | 18/40(45%) | 4.013 |
| P16 | 2.00(0–753.36) | 0(0–2.90) | 0(0–0) | 18/40(45%) | 2.9 |
| DcR1 | 0.19(0–72.44) | 0(0–0.96) | 0(0–0) | 9/40(23%) | 0.96 |
| DcR2 | 0(0–481.94) | 0(0–4.37) | 0(0–0) | 11/40(28%) | 4.37 |
| APC | 4.1(0–331.13) | 0.29(0–13.95) | 0(0–0) | 12/40(30%) | 13.95 |
| DAPK | 0.10(0–4.70) | 0(0–0.22) | 0(0–0) | 17/40(43%) | 0.22 |
| SOCS-1 | 2.72(0–111.39) | 0(0–6.3) | 0(0–16) | 13/40(33%) | 6.3 |
| H-CAD | 2.92(0–148.94) | 0(0–7.59) | 0(0–3.6) | 11/40(28%) | 7.58 |
| SOCS-3 | 1.88(0–912.04) | 0.21(1–186.2) | 0(0–11.73) | 2/40(5%) | 186.2 |
| E-CAD | 3.98(0–467.7) | 2.56(0–39.8) | 0(0–18.84) | 5/40(12%) | 39.8 |
Cutoff value=the highest QR value in adjacent non-malignant lung. For SOCS3 and E-CAD cutoff value is too high and thus no meaningful conclusion can be drawn about % samples+ve for methylation in NSCLC.