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Expression analysis of INK4, Cdk4, and Cdk6 in glioblastoma multiforme (GBM) xenograft panel. Archived flank tumor specimens from 3 individual mice for each tumor line were processed for Western blotting and RT-PCR. Protein and RNA samples from each animal were pooled at equal ratio and used for analysis. (A) Western blot analysis of p16INK4a, Cdk4, and Cdk6 expression. (B) RT-PCR using human-specific primers for CDKN2A, CDKN2B, CDKN2C, and CDKN2D.
