Fig. 2.

RT-PCR of ESTs identified via SSH. Negative gel images of 25 RT-PCR reactions performed confirm differential expression of ESTs detected by SSH (see Tables 1, 2; supplementary material Table S1). For each RT-PCR reaction, the number listed corresponds to primers and sequence information listed in Table 1 or Table 2. RT-PCR products are grouped by putative cell functions (explained in Materials and methods). Sequences labeled in blue are upregulated in EO, sequences in gray are upregulated in SM when compared to actin control. ^†ESTs that matched different regions of the same Danio rerio sequence during BLAST searching (see Materials and methods). *ESTs that SSH revealed as differentially expressed in one library that were later found via RT-PCR to be differentially expressed in the opposite library. All PCR products matched expected sizes listed in Tables 1 and 2.