Table 2.
Stresses performed on female-sterile strains S1-B12 and S3-A10 and the corresponding wild-type strains S1 and S3
| Proportion of female-fertile replicates before treatment | Proportion of female-fertile replicates after treatments | |||||||
| Stress | S1 | S3 | S1-B12 | S1-A10 | S1 | S3 | S1-B12 | S3-A10 |
| -80°C (1 h) |
5/5 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 | 0/5 | 0/5 |
| -80°C (24 h) |
5/5 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 | 0/5 | 0/5 |
| Mycelium mashing |
5/5 | 5/5 | 0/5 | 0/5 | 5/5 | 5/5 | 0/5 | 0/5 |
| single-spore isolation |
1/1 | 1/1 | 0/1 | 0/1 | 12/12 | 12/12 | 0/12 | 0/10 |
| growth on plant | 1/1 | 1/1 | 0/1 | 0/1 | 7/10 | 10/10 | 0/8 | 0/10 |
The treatments were: one hour at -80°C, 24 hours at -80°C, mycelium mashing by ultrasound, single-spore isolation and growth on plants. The ratios (number of replicates in which the strain was female-fertile)/(number of replicates in which the strain was female-sterile) are given before and after the various treatments