| 1 cycle: | 5 minutes | 95°C | (initial denaturation) |
| 35 cycles: | 30 seconds | 95°C | (denaturation) |
| 30 seconds | 55°C | (annealing) | |
| 30 seconds | 72°C | (extension) | |
| 1 cycle: | 5 minutes | 72°C | (final extension) |
| 1 cycle: | indefinitely | 4°C | (hold) |
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