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. 2012 Jun 20;7(6):e39505. doi: 10.1371/journal.pone.0039505

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Kalinichenko et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Nedd4 in ubiquitin ligase-independent manner rescue axis extension defects in Xenopus development induced by MAK-V overexpression. Two ng each of MAK-V, Nedd4, Nedd4(CS) mRNA was injected alone or together as indicated into two dorso-animal blastomeres at 4–8 cell stage. Embryos were fixed at stage 38.