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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Hepatology. 2012 Jun 6;56(1):219–227. doi: 10.1002/hep.25662

Figure 5. Tregs modulate the molecular phenotype of hepatic DCs in neonatal mice.

Figure 5

(A) Hepatic pan-DCs purified from RRV infected mice at 7dpi were co-cultured with Tregs (CD25+) or non-Treg (CD25−) CD4 lymphocytes from non-infected mice and subjected to flow cytometry after 72 hours. The numbers in the dot plots denote frequencies of CD11b+ mDCs expressing CD86. The bar graph displays the fold change of frequency of CD86+ mCDs in presence of Tregs or non-Treg CD4 cells compared with DCs cultured without CD4 cells, denoted by the dashed line, from 2 independent experiments. (B) Hepatic MNCs were isolated from neonatal mice on day 7 after RRV challenge preceded by AT of CD4 cells (n=4), AT of CD25−CD4 cells (n=5) or no AT (n=7), and subjected to flow cytometry to enumerate total numbers of hepatic mDCs. Representative dot plots are shown in Supplemental Fig 6. (C) Expression of CD86 on hepatic mDCs was determined by flow cytometry and is displayed as mean fluorescent intensity (MFI) for CD86. Values in (B) and (C) are expressed as mean+SEM with * p<0.05, comparing the three groups using one-way ANOVA. Differences not denoted with * are non-significant.