Figure 7. Depletion of Tregs in older mice increases susceptibility to RRV induced hepatobiliary injury.
8-day-old mice were challenged with RRV following Treg-depletion with αCD25-antibody or treatment with isotype-matched IgG. (A) Plasma total bilirubin and ALT concentrations at 12dpi were determined in colorimetric assays. Values are expressed as mean+SEM with * p<0.05, in unpaired t test. (B) EHBDs were microdissected from RRV-infected αCD25- or IgG-treated mice at 12 dpi and stained with H&E. Representative photomicrographs of longitudinal sections of the EHBDs near the liver hilum demonstrate increased peri-ductal inflammation and narrowing of the lumen (*) in CD25-depleted mice compared with controls.