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Dependence of the second-order rate constants (kcat/Km) for the TIM-catalyzed turnover of the carbonyl form of [1-13C]-GA in D2O on the concentration of HPO32- at pD 7.0 and 25 °C (I = 0.1, NaCl). The data were fit to eq 9 derived for the model shown in Scheme 5. (A) (●) L232A TbbTIM, (■) wildtype TbbTIM,39 (◆) I172V TbbTIM. (B) I172A TbbTIM.
