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. Author manuscript; available in PMC: 2013 Jun 20.
Published in final edited form as: J Am Chem Soc. 2012 Jun 6;134(24):10286–10298. doi: 10.1021/ja303695u

Table 4.

Kinetic Data for the Reaction of [1-13C]-GA Catalyzed by the I172A mutant of TbbTIM in D2O in the Absence and Presence of Phosphite Dianion in D2O at 25 °C.a

[HPO32-], mM [TIM], M kobs, (s-1)b (kcat/Km)obs (M-1s-1)c (kcat/Km) (M-1s-1)d
0 3.1 × 10-4 7.5 × 10-7 0.040
0 3.4 × 10-4 1.1 × 10-6 0.055
0 7.3 × 10-4 1.8 × 10-6 0.041
2.5 4.0 × 10-4 2.3 × 10-6 0.096 0.055
5 3.4 × 10-4 2.5 × 10-6 0.12 0.071
5 6.9 × 10-4 5.3 × 10-6 0.13 0.071
5 3.3 × 10-4 2.4 × 10-6 0.12 0.067
7.5 4.0 × 10-4 4.1 × 10-6 0.17 0.096
10 1.7 × 10-4 2.0 × 10-6 0.19 0.11
10 3.4 × 10-4 3.6 × 10-6 0.18 0.10
10 4.0 × 10-4 4.6 × 10-6 0.19 0.11
12.5 4.0 × 10-4 5.3 × 10-6 0.22 0.12
15 1.7 × 10-4 2.4 × 10-6 0.23 0.13
15 2.1 × 10-4 3.1 × 10-6 0.24 0.14
15 4.0 × 10-4 5.6 × 10-6 0.23 0.13
17.5 4.0 × 10-4 5.9 × 10-6 0.24 0.14
20 1.5 × 10-4 2.40 × 10-6 0.26 0.15
20 1.7 × 10-4 2.7 × 10-6 0.26 0.15
20 1.7 × 10-4 2.5 × 10-6 0.25 0.14
20 4.0 × 10-4 6.4 × 10-6 0.26 0.15
a

For reactions of 20 mM [1-13C]-GA at pD 7.0 and I = 0.1 (NaCl).

b

Observed first-order rate constant for the reaction of [1-13C]-GA calculated using eq 1.

c

Observed second-order rate constant for the TIM-catalyzed reaction of [1-13C]-GA calculated using eq 2.

d

Second-order rate constant for the reactions of [1-13C]-GA, calculated (eq 8) from (kcat/Km)obs and the average product yield fE = 0.57 ± 0.04 calculated from the product data reported in Table 3.