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. Author manuscript; available in PMC: 2013 Jun 20.
Published in final edited form as: J Am Chem Soc. 2012 Jun 6;134(24):10286–10298. doi: 10.1021/ja303695u

Table 5.

Kinetic Parameters for the Phosphite-Activated and Unactivated Reactions of [1-13C]-GA Catalyzed by Wildtype Tbb and Mutant TIMs in D2O at 25 °C.a,b

TIM (kcat/Km)E (M-1 s-1) (kcat/Km)E•HPi (M-1 s-1) Kd (mM) (kcat/Km)E•HPi/(kcat/Km)E (kcat/Km)E•HPi/Kd (M-2 s-1)
WT Tbbc 0.07 64 19 900 3400
L232Ad 1.2 100 1.2 80 83000
I172V 0.03 27 16 900 1700
I172A < 0.003e 0.23 12 > 77 20
a

For reactions at pD 7.0 and I = 0.1 (NaCl).

b

The kinetic parameters are defined in Scheme 5.

c

Data from ref 39.

d

Data from ref 31.

e

Limit calculated as described in the text.