Figure 1.

Viability of cells expressing CTD mutants. (A) Overview of analysed CTD mutants. (B) HEK293 cells were transfected with an expression vector for Rpb1 carrying a point mutation for α-amanitin resistance (wild type). CTD mutants carry 48 consensus repeats (Con48), or have replaced specific residues in all consensus repeats by alanine (Ser2, Thr4, Ser5, Ser7) or serine (Thr4). Twenty-four hours after transfection, α-amanitin was added (2 μg/ml) and cell growth rates were monitored for 4 days in the xCELLigence system (Roche), error bars show standard deviation of 3 replicates.