Impaired AM phagocytosis in CCR2−/− mice after LC. Phagocytosis of non–serum opsonized FITC labeled Pseudomonas aeruginosa was assessed using AMs from uninjured mice, as well as injured WT and CCR2−/− mice, as described in Materials and Methods (mean ± SEM; n = 14 per group). For possible differences in AM adherence to tissue culture plate, data were normalized for cell number using a LDH Cytotoxicity Detection Kit (Roche, Indianapolis, IN). Statistical analysis was performed on data at each time point, and paired samples were analyzed using the two-tailed unpaired t test with Welch's correction. *P < 0.05 compared with corresponding WT.