Figure 7.

Neutrophil levels in the BAL were higher in the CCR2^−/− mice after LC. Flow cytometric analyses of relative numbers of neutrophils in the BAL at 5, 24, 48, and 72 hours after lung injury based on CD11b and GR1 expression (A). Cytospin analysis of BAL samples collected at 5, 24, 48, and 72 hours after lung injury (B), as described in detail in Materials and Methods. Values are presented as means (±SEM; n = 14). *P < 0.05 compared with corresponding WT. (C) Histopathology of Lung in CCR2^−/− and WT mice a 5, 24, 48, and 72 hours after LC are shown at 400× magnification.