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. 2012 Jun;46(6):797–806. doi: 10.1165/rcmb.2011-0358OC

Figure 8.

Figure 8.

Recruitment of AMs was significantly less in CCR2−/− mice after LC. (A) Cytospin analysis of BAL samples collected at 5, 24, 48, and 72 hours after lung injury visualized by light microscopy at ×200 magnification. Values are represented as mean and SEM (n = 14). (B) Flow cytometric analyses of relative numbers of monocytes/ macrophages and neutrophils in the BAL at 5, 24, 48, and 72 hours after lung injury based on CD11b and CD11c expression and the presence of MHC II. Values are represented as mean and SEM (n = 14). *P < 0.05 compared with corresponding WT. (C) This is an example of FCM analysis of BAL macrophages and neutrophils after LC. Cells collected by BAL after LC were stained for five -color fluorescence and the macrophages and neutrophils subset (defined by forward and side scatter) was selected for FACScan analysis.