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GPCR and signaling protein expression in ASM. (A–C) Expression of bradykinin B2 (A), histamine H1 (B), or PAR1 (C) receptors was determined by immunoblotting. *P = 0.04, Mann-Whitney U test. (D) Relative expression of signaling proteins in control and asthmatic ASM cells was determined by densitometry (normalized to β-actin, n = 3–7 donors/group). WB, Western blot.
