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Lower density of GFAP immunostaining in aged E2 rats. A, Optical densitometry confirmed a significantly reduced density of GFAP immunostaining in ischemic cortex of aged E2 rats compared with both intact and OVX counterparts. B, No significant differences in immunostaining density of Iba-1 were observed in the ischemic cortex of the aged treatment groups. *, P < 0.001 compared with intact; †, P < 0.001 as compared with OVX.
