Table 1.
Primers used for real-time PCR
| Gene symbol | Sequence | References |
|---|---|---|
| Glut1 | Forward, CCAGCTGGGAATCGTCGTT | 38 |
| Reverse, CAAGTCTGCATTGCCCATGAT | ||
| Glut3 | Forward, CTCTTCAGGTCACCCAACTACGT | 38 |
| Reverse, CCGCGTCCTTGAAGATTCC | ||
| Snat1 | Forward, GATTTACAGGACATTTTTGACCACATAGTA | 39 |
| Reverse, CACCTGTACTAGAAGGTGGACCATTT | ||
| Snat2 | Forward, AGAAGCCATCTTTGATCAAATTGTG | 39 |
| Reverse, GACCTGAACTAGAAGGTGAATCATGC | ||
| Snat4 | Forward, TCACACTGCTGTTTCCAAGG | a |
| Reverse, CAGCCGGAAGAATGAAAATC | ||
| Slc7a3 | Forward, TTCTGGCCGAGTTGTCTATGTTTG | c |
| Reverse, AGTGCGGTTCTGTGGCTGTCTC | ||
| 11betaHsd2 | Forward, CTGCAGATGGATCTGACCAA | 40 |
| Reverse, GTCAGCTCAAGTGCACCAAA | ||
| Igf2 | Forward, AAGAGTTCAGAGAGGCCAAACG | 38 |
| Reverse, CACTGATGGTTGCTGGACATCT | ||
| igf2P0 | Forward, CCGAGGCCTGTACCACCTA | 21 |
| Reverse, CCTCGGCTCAGACCTCAGTA | ||
| H19 | Forward, AATGGTGCTACCCAGCTCAT | a |
| Reverse, GCAGAGTTGGCCATGAAGAT | ||
| Gapdh | Forward, ACAACTTTGGCATTGTGGAA | b |
| Reverse, GATGCAGGGATGATGTTCTG |
a
Designed using the Primer3 web program http://frodo.wi.mit.edu/primer3/.
b
Designed using the PrimerSelect, DNASTAR lasergene software (Madison, WI).
c
Designed using PerlPrimer program http://perlprimer.sourceforge.net/.