Table 1.

The primer sequences of the oligonucleotides used for ChIP, RT-PCR, qRT-PCR, and cloning

Name	Gene ID	Application	Sequences
Human hepcidin, first from the left of Fig. 1A	57817	ChIP	Forward, 5′-CAGTGGTTGGTTGGATGGAT
			Reverse, 5′-TTGTGGGAAGAGACGAGTGC
Human hepcidin second site	57817	ChIP	Forward, 5′-AACCGGCTCCCCAAAAAC
			Reverse, 5′-CCCCGTCGGACACTCATT
Human hepcidin third site	57817	ChIP	Forward, 5′-CGCCGTGTGGAGTCTTATTC
			Reverse, 5′-GCTTATGGGGGCTTCCTCT
Human hepcidin fourth site	57817	ChIP	Forward, 5′-GTCCCCTCCCTTCCTTATTT
			Reverse, 5′-CTTTCATCCCCTATTATTGCAAC
Human hepcidin	57817	RT-PCR	Forward, 5′-CCTGACCAGTGGCTCTGTTT
			Reverse, 5′-CACATCCCACACTTTGATCG
Human GAPDH	2597	RT-PCR	Forward, 5′-TGGTATCGTGGAAGGACTC
			Reverse, 5′-AGTAGAGGCAGGGATGATG
Mouse GAPDH	14433	qPCR	Forward, 5′-GGCATTGCTCTCAATGACAA
			Reverse, 5′-CCCTGTTGCTGTAGCCGTAT
Mouse hepcidin-1	84506	qPCR	Forward, 5′-AGAAAGCAGGGCAGACATTG
			Reverse, 5′-GATGCAGATGGGGAAGTTGG
Mouse hepcidin-2	66438	qPCR	Forward, 5′-AGAAAGCAGGGCAGACATTG
			Reverse, 5′-TCTGCAGATGGGGAAGTTGA
Human hepcidin promoter (3171 bp)	57817	Luciferase activity	Forward, 5′-TCTCGAGCTCAAGCTTGTCTGTCTGTCGCCCTTCTC
			Reverse, 5′-GGCGACCGGTGGATCCGTCTGGGACCGAGTGACAGT
Human hepcidin promoter	57817	ERE deletion	Forward, 5′-AGGGTGTGGGGAGCTGGAGGACATGTCCCATGTTG
			Reverse, 5′-CAACATGGGACATGTCCTCCAGCTCCCCACACCCT
Human hepcidin probe with WT ERE	57817	EMSA	Forward, 5′-AGGGTGTGGGGAGCTGGGGTCAAGGACATGTCCCATGTTG
			Reverse, 5′-CAACATGGGACATGTCCTTGACCCCAGCTCCCCACACCCT
Human hepcidin probe with mutated ERE	57817	EMSA	Forward, 5′-AGGGTGTGGGGAGCTGGAAAAAAGGACATGTCCCATGTTG
			Reverse, 5′-CAACATGGGACATGTCCTTTTTTCCAGCTCCCCACACCCT
TfR1	22042	qPCR	Forward, 5′-CATGAGGGAAATCAATGATCG
			Reverse, 5′-ACATAGGGCGACAGGAAGTG
DMT-1	18174	qPCR	Forward, 5′-TGGGTCTGTCTTTCCTGGAC
			Reverse, 5′-CATCCACGGTGTTCAGAAGAT