Fig. 1.

Alignment of human, mouse, African green monkey, bullfrog, leopard gecko, chicken, zebrafish, and lamprey GnRH receptor sequences. The sequences (see Fig. 5, accession details) were aligned with ClustalW2 (28), and an image was generated by editing and annotating, the style based on the output initially produced with BOXSHADE 3.21. Ubiquitously conserved amino acid residues are shown with dark background shading, and amino acids with conserved substitutions are shown with light shading Semiconserved substitutions are shown with a dot on the consensus line. Bold horizontal lines indicate predicted TMD (TMD 1–7). GnRH receptor subtypes are labeled with ordinal numbers according to the published terminology (7, 14, 47, 50) and grouped together. Residues, microdomains, and motifs discussed in the text are bracketed above and below the alignments.