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. 2012 Jun 21;18(23):2938–2947. doi: 10.3748/wjg.v18.i23.2938

Figure 3.

Figure 3

Expression of toll-like receptor 4 mRNA in hepatocellular carcinoma patients and hepatoma cell lines. A: Reverse transcriptase polymerase chain reaction analysis of total RNA from hepatocellular carcinoma (HCC) and normal tissues. The toll-like receptor 4 (TLR4) mRNA expression in HCC tissues was stronger (2.08 ± 0.14) compared to normal tissues (0.48 ± 0.05) (P = 0.01); B: TLR4 mRNA expressed in human HepG2 and murine H22 hepatoma cell lines; C: Expression of TLR4 protein in HCC and normal tissues by immunohistochemistry. a, c: Paraffin-embedded sections of HCC exhibiting positive expression on the membrane and in the cytoplasm in several positive hepatocytes (arrows) [diaminobenzidine (DAB) ×400]; b, d: Paraffin-embedded sections of normal tissues showing weaker expression (DAB ×400); D: TLR4 was detected by immunohistochemistry in tissue sections of patients with HCC (frequency of positive hepatocytes for TLR4 in paraffin-embedded sections).