Table 1.

Methyl thiazolyl tetrazolium assay

Cell line	Optical density value
SMMC-7721	1.77 ± 0.20
pEGFP-Nl/SMMC-7721	1.78 ± 0.13
pEGFP-Nl-WWOX/SMMC-7721	1.12 ± 0.23

The cell growth of parental SMMC-7721, control vector and WWOX over-expressing cells was examined by methyl thiazolyl tetrazolium assay over a 3-d period. The cell growth of the WWOX expressing cells (pEGFP-N1-WWOX/SMMC-7721) was reduced compared with the wild-type (SMMC-7721) and control vector (pEGFP-N1/SMMC-7721) cells (P < 0.05).