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. 2012 Jun 21;3:220. doi: 10.3389/fphys.2012.00220

Figure 1.

Figure 1

Effects of cyclic nucleotide or Cx43 stimulation on VSMC proliferation. (A) In rat primary VSMCs in the presence of 10% serum for 72 h and using the MTT assay, 8Br-cAMP (100 μM), the sGC stimulator BAY (100 nM) or the garlic-derived Cx inducer DADS (50 μM) significantly reduced rat primary VSMC numbers compared with vehicle controls. Data are mean ± SEM with an n = 6. (B) Using hemocytometry after 72 h, both 8Br-cAMP and BAY significantly reduced cell numbers by ∼40%, and the presence of DADS reduced cell numbers by ∼20% (p = 0.06). Data are mean ± SEM of two independent experiments each with n = 3–4. (C) MTT assay performed on VSMCs after 12 h shows no observable changes in any treatment group. Student–Newman–Keuls method for multiple comparisons following one-way ANOVA was used. *p < 0.05 compared with normalized vehicle controls.