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Effects of 8Br-cAMP and DADS on 10% FBS-stimulated VSMC cell cycle progression. Using flow cytometry after 16 h incubation, a 40% reduction in the percentage of cells in the S-phase was observed in the presence of DADS (50 μM) with no significant changes in the G0/G1 or G2/M phases compared with vehicle controls. Data are mean ± SEM with an n = 3. Student–Newman–Keuls method for multiple comparisons following one-way ANOVA was used. *p < 0.05compared with vehicle controls.
