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. 2012 Jun 21;3:220. doi: 10.3389/fphys.2012.00220

Figure 5.

Figure 5

Results from in-cell Western analyses showing effects of kinase inhibition on total Cx43 expression in rat primary VSMCs following incubation in the presence of (A) 8Br-cAMP (100 μM), (B) 8Br-cGMP (100 μM), (C) BAY (100 nM), and (D) DADS (50 μM) for 270 min. (A) Inhibition of PKA (by PKI), PKG (by DT2), or PKC (by CalC) each independently subdued 8Br-cAMP-induced increase in Cx43 expression. (B,C) Only PKG and PKC inhibition reversed the effects of 8Br-cGMP and BAY, respectively. (D) Stimulation of Cx43 expression in the presence of DADS was not dependent on PKA, PKG, or PKC. (E) Treatment with any individual kinase inhibitor did not affect total basal Cx43 expression compared with vehicle controls. Data are mean ± SE from n = 4–7 for each treatment. Student–Newman–Keuls method for multiple comparisons following one-way ANOVA was used. *p < 0.05 compared with normalized vehicle controls; #p < 0.05 comparing inhibitors to agonists alone normalized to vehicle controls.