Figure 7.

Effect of kinase inhibition on (A) 8Br-cAMP- and (B) DADS-stimulated GJIC estimated by FRAP. Rat primary VSMCs were incubated for 15 min in the presence of the following PKA, PKG, and PKC inhibitors: PKI (10 μM), DT-2 (10 μM), and Calphostin-C (100 nM), respectively, followed by 8Br-cAMP (100 μM) or DADS (50 μM) alone for 15 min. (A) Stimulation of GJIC by 8Br-cAMP was effectively inhibited by PKC inhibition alone but not by inhibition of PKA or PKG. (B) DADS-mediated GJIC stimulation was partially (non-significantly) reversed by PKA or PKG inhibition. Data are mean ± SE from an n = 4 for the inhibitors treatment and an n = 6–9 for control, 8Br-cAMP and DADS. Student–Newman–Keuls method for multiple comparisons following one-way ANOVA was used. *p < 0.05 compared with normalized vehicle controls, ^#p < 0.05 compared with cAMP-treated cells.