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. 2012 Jun 21;3:220. doi: 10.3389/fphys.2012.00220

Figure 8.

Figure 8

In-cell Western analyses of site-specific Cx43 phosphorylation in rat primary VSMCs in the presence of (A) 8Br-cAMP and (B) DADS. (A) In the presence of 8Br-cAMP, phosphorylation of the MAPK-sensitive Ser279 was significantly increased compared to vehicle controls. (B) The presence of DADS significantly increased phosphorylation at the MAPK sensitive Ser255 and Ser279 sites, p34cdc2 kinase-sensitive Ser262, and the PKC-sensitive Ser368. Data are mean ± SE from three independent experiments with an n = 4 for Ser279 phosphorylation, and n = 3–4 for Ser255, 262, and 368 phosphorylation. Student–Newman–Keuls method for multiple comparisons following one-way ANOVA was used. *p < 0.05 compared with respective vehicle controls.