Table 2.
Microscopic rate constants for the loop dynamics in YopH and its Q357A/F mutants at different final T-jump temperatures.
| YopH Q357F | kclose(ms−1) | kopen(ms−1) | kclose/kopen | koff/kon(μM) | ||||
|---|---|---|---|---|---|---|---|---|
| 33 °C | 98±18 | 6±1 | 16 | 821±22 | ||||
| 28 °C | 58±9 | 4±1 | 15 | 484±17 | ||||
| 23 °C | 39±6 | 2.6±0.4 | 15 | 322±13 | ||||
| 13 °C | 19±3 | 1.1± 0.1 | 17 | 175±10 | ||||
|
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| YopH WT | ||||||||
|
| ||||||||
| 33 °C | 230±20 | 16±1 | 14 | 1150±200 | ||||
| 28 °C | 139±9 | 11±1 | 13 | 700±100 | ||||
| 23 °C | 91±4 | 8±1 | 11 | 450±50 | ||||
| 13 °C | 46±3 | 3±1 | 15 | 250±50 | ||||
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| YopH Q357A | ||||||||
|
| ||||||||
| 33 °C | 178±20 | 16±3 | 11 | 496±29 | ||||
| 28 °C | 138±18 | 14±2 | 10 | 469±31 | ||||
| 23 °C | 108±15 | 10±2 | 11 | 442±28 | ||||
| 13 °C | 84±12 | 6±1 | 14 | 398±21 | ||||
The values of kclose, kopen and koff/kon are deduced from the curve fit of the data in Figure 7 to Equation 4. Their uncertainties are the RMSDs of the fits.