. Author manuscript; available in PMC: 2012 Oct 15.

Published in final edited form as: J Neurosci Methods. 2011 Aug 12;201(2):390–398. doi: 10.1016/j.jneumeth.2011.08.011

Table 1.

Number of animals enrolled per group.

	Baseline sex difference studies
Strain	C57Bl/6^a				B6129Sf/J
Male	19				12
Female	53				18
	Morphine effect studies
Morphine dose	0 μg/mouse	10 μg/mouse	20 μg/mouse	30 μg/mouse	40 μg/mouse
Male	3	4	2	6	4
Female	16	6	8	10	13
	Isoflurane anesthesia study
Anesthesia	Sham Anesthesia				Isoflurane anesthesia
Male	8				8
Female	8				8

For baseline sex difference studies in C57Bl/6 mice, we included all baseline measurements obtained in animals from the morphine study before any intervention was made. Animals in the morphine study were given only one of the 5 morphine doses. Only C57Bl/6 mice were included in the morphine effect study.