Table 1. Primers used for the amplification and sequencing of A2AR.
| Fragment | Direction | Primer sequence | PCR product (bp) | Exon |
|---|---|---|---|---|
| A2A1 |
S |
5′- CGCCCTCTGCAGATGGTTCAGCT-3′ |
428 |
1 |
| |
AS |
5′- GTTGCTGTTGAGCCACACGGCC −3′ |
|
|
| A2A2 |
S |
5′- CCGTGCTGAGCCTGCCTGTCGTC −3′ |
434 |
2 |
| |
AS |
5′- CCTGGCTCCGGGCACAGACCAA −3′ |
|
|
| A2A3 |
S |
5′- CCCTGGCTTCTCAGATCTCTGAT −3′ |
577 |
2 |
| |
AS |
5′- GGCGTAGATGAAGGGATTCACA −3′ |
|
|
| A2A4 |
S |
5′- CTCATGTACCTGGCCATCGTCCTC −3′ |
611 |
2 |
| AS | 5′- CTTCTTGCTGGGCCTCATGCTG −3′ |
S, sense strand; AS, antisense strand.