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Southern blot of digested Rotylenchulus reniformis genomic DNA probed with a DIG-labeled Rr-eng-1 cDNA. Examples of hybridizing bands are indicated by arrows in lanes 2 and 5. 10 μg digested DNA were loaded in lanes 2-7. Lane 1 (DIG marker), lane 2 (EcoRI), lane 3 (HindIII), lane 4 (BamHI + EcoRI), lane 5 (EcoRI + HindIII), lane 6 (BamHI + HindIII), and lane 7 (BamHI + EcoRI + HindIII).
